کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889341 | 1080826 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid detection of ricin in cosmetics and elimination of artifacts associated with wheat lectin
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Ricin can be detected in cosmetics at 0.005 µg/mL in the analytical sample using lateral flow devices (LFDs). Wheat germ, an ingredient used in skin care products is also a potential source of wheat lectin. False positives were observed when wheat lectin was added to LFDs from two manufacturers, irrespective of whether the LFD was specific for ricin, Staphylococcus enterotoxin B (SEB), or botulinum toxin. In contrast, pea and peanut lectins did not cause false positives. Substitution of the buffer supplied with the LFDs with a buffer containing 2.5% non-fat milk powder eliminated the occurrence of false positives. This substitution increased the LOD to 0.01 µg/mL ricin, which is an acceptable level for screening cosmetics for contamination by ricin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 336, Issue 2, 31 July 2008, Pages 251-254
Journal: Journal of Immunological Methods - Volume 336, Issue 2, 31 July 2008, Pages 251-254
نویسندگان
Jacqueline Dayan-Kenigsberg, Agnès Bertocchi, Eric A.E. Garber,