کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889567 | 1081473 | 2013 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni Molecular methods to investigate adhesion, transmigration, invasion and intracellular survival of the foodborne pathogen Campylobacter jejuni](/preview/png/10889567.png)
چکیده انگلیسی
Campylobacter jejuni is a spiral-shaped Gram-negative pathogen and major agent of gastrointestinal foodborne illness in humans worldwide. This pathogen encodes numerous described pathogenicity-associated factors involved in important processes including bacterial adhesion to, transmigration across, invasion into and intracellular survival within intestinal epithelial cells. This review article highlights various molecular techniques applied in the studies of each of these individual steps of C. jejuni host cell interactions in vitro including gentamicin protection assay, chemotaxis and motility assays, transwell and intracellular survival assays, G-Lisa, siRNA knockdown, immunohistochemistry, immunofluorescence, electron microscopy and luciferase reporter assays. We discuss the strengths and limitations of the methods as well as the different cell model systems applied. Future work should employ new technologies including modern microscopic, proteomics-based and cell signaling approaches to identify and characterise novel virulence mechanisms, which are crucial to provide fresh insights into the diversity of strategies employed by this important pathogen to cause disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 95, Issue 1, October 2013, Pages 8-23
Journal: Journal of Microbiological Methods - Volume 95, Issue 1, October 2013, Pages 8-23
نویسندگان
Steffen Backert, Dirk Hofreuter,