Combining COLD-PCR and high-resolution melt analysis for rapid detection of low-level, rifampin-resistant mutations in Mycobacterium tuberculosis

► COLD-PCR/HRM is developed for detecting low-level mutations in M. tuberculosis. ► It shows better selectivity for low-level mutation compared to traditional method. ► COLD-PCR/HRM offers greater resolution for detecting drug-resistant M. tuberculosis.