A novel high-resolution melting analysis-based method for Yersinia pseudotuberculosis genotyping

► A method of Yersinia pseudotuberculosis genotyping based on HRMA was developed ► 10 SNPs were screened from sequences in the 16S rRNA, glnA, gyrB and recA genes ► Y. pseudotuberculosis were separated into two groups based on bio-serogroups ► This assay may be used to genotype Y. pseudotuberculosis of O:1 and O:3 serogroups ► This rapid and cost-effective method can complement sequence-based methods