Rapid screening of an ordered fosmid library to clone multiple polyketide synthase genes of the phytopathogenic fungus Cladosporium phlei

► We describe an efficient arrangement of an ordered fosmid library of C. phlei. ► PCR screening of the mixed clones of an ordered library can identify the PKS genes. ► Characterization of four PKS genes representing all fungal subclasses is presented.