Rapid quantification of pathogenic fungi by Cellometer image-based cytometry

► Image-based cytometric methodology for the quantification of viable pathogenic yeasts ► Compared fungal viability as assessed by Cellometer image-based cytometry versus colony forming units. ► Rapid method to measure proliferation of Histoplasma capsulatum within RAW 264.7 macrophages ► Rapid method to quantify relative amounts of GFP-expressing Candida albicans within live macrophages ► Rapid method to study cytotoxicity induced by antifungal drugs.