Simplified detection of food-borne pathogens: An in situ high affinity capture and staining concept

► Direct detection of food-borne pathogens in primary enrichment broth. ► Use of non-fouling surface with enhanced receptor coating ► High affinity surface using very specific phage tail proteins. ► Triphenyltetrazolium chloride as intracellular dye for easy visual reading. ► Applied to three pathogens: E. coli O157:H7, Salmonella spp. and Listeria spp.