Design and validation of a novel quantitative method for rapid bacterial enumeration using programmed stage movement scanning electron microscopy

► The method for detecting bound bacteria is an important consideration in the study of bacterial adherence. ► We have developed and validated a method for enumerating bacteria on porous meshes. ► Cumulative counts after 1-10 stage movements were compared to absolute bacterial counts. ► Results demonstrated significant correlation to the absolute count after five cumulative counts.