Development of a Lactobacillus specific T-RFLP method to determine lactobacilli diversity in complex samples

► We developed a novel T-RFLP protocol based on Lactobacillus hsp60. ► We determined the terminal restriction fragment (TRF) profiles of 96 Lactobacillus spp. or strains. ► This new protocol is semi-quantitative. ► We performed this protocol on mice fecal samples for community and species level analysis.