کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889647 | 1081482 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessment of Candida shehatae viability by flow cytometry and fluorescent probes
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Quantification of different physiological states of Candida shehatae cells was performed by flow cytometry associated with two fluorescent probes. Propidium iodide and carboxyfluorescein diacetate acetoxymethyl ester fluorescent dyes were chosen based on data from the literature. A staining procedure, developed from the previous works was applied to the yeast. Then, the protocol was improved to fit with fermentation constraints such as no physiological interference between the staining procedure and the cells, shortest preparation time and small amounts of dyes. From this optimisation, propidium iodide was included in the sample at 8 mg/L whereas carboxyfluorescein was first diluted in Pluronic® agent and used at 3 mg/L, samples were incubated for 10 min at 40 °C. Repeatability and accuracy were evaluated to validate this flow cytometry procedure for viability determination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 91, Issue 1, October 2012, Pages 8-13
Journal: Journal of Microbiological Methods - Volume 91, Issue 1, October 2012, Pages 8-13
نویسندگان
Julie Monthéard, Sandy Garcier, Eric Lombard, Xavier Cameleyre, Stéphane Guillouet, Carole Molina-Jouve, Sandrine Alfenore,