BAPJ69-4A: A yeast two-hybrid strain for both positive and negative genetic selection

► We describe the creation of a novel yeast two-hybrid strain, BAPJ69-4A. ► BAPJ69-4A contains four markers: ADE2, HIS3, URA3, and lacZ. ► The addition of the URA3 marker allows for both positive and negative selection. ► The differences in marker stringency allow for highly tunable assay sensitivity. ► BAPJ69-4A was characterized using the retinoid X receptor and variants.