Optimization of the URA-blaster disruption system in Candida guilliermondii: Efficient gene targeting using the URA3 marker

► A simple transformation system for C. guilliermondii was established. ► An ATCC 6260-derived uracil auxotroph strain and a URA3 blaster cassette were developed. ► This strategy allows efficient multiple gene disruption by homologous recombination.