A modified acid-fast staining method for rapid detection of Mycobacterium tuberculosis

► Modified IK acid-fast staining or ZN acid-fast staining method is time consuming. ► A new, modified staining method is described. ► In which carbol fuchsin and dioxogen are added to the slide. ► The method resulted in shortened processing time and eliminated need for heating. ► The method for detection of M. tuberculosis was sensitive, reliable, and rapid.