Gene transfection of Toxoplasma gondii using PEI/DNA polyplexes

► PEI/DNA polyplex is capable of transfecting GFP gene to Toxoplasma gondii. ► GFP-expressing tachyzoites and bradyzoites are observed in infected HFF cells. ► Effective and inexpensive reagent to ferry GFP-encoded plasmids into T. gondii. ► Potential tool for studying parasite interconversion stages and drug screening.