Optimal method for efficiently removing extracellular nanofilaments from Shewanella oneidensis MR-1

► Optimized shearing methods for preparing Shewanella oneidensis MR-1 nanofilaments ► Atomic force microscope imaging of flagella and pili. ► Identification of potential bacterial nanowire proteins ► Vortexing for 5 or 10 min produced the best nanofilament samples