Use of 3-hydroxy fatty acid concentrations in a murine air pouch infection model as a surrogate marker for LPS activity: A feasibility study using environmental Burkholderia cenocepacia isolates

► We suggested B. cenocepacia was recognized as good candidate for the air pouch study. ► C14:0 3-OH FA and endotoxin unit levels can be used as surrogates for LPS concentration. ► Both surrogates were correlated in a dose-dependent manner in air pouch samples. ► Both surrogates had different effects on NO and TNF-α production by the cells. ► C14:0 3-OH FA, rather than endotoxin unit, acted as a stimulatory indicator for LPS.