Purification of modified mycobacterial A60 antigen by affinity chromatography and its use for rapid diagnostic tuberculosis infection

► A new affinity column was designed to prepare modified A60-Ag compared to unmodified Ag to prepare Abs for the TB-Rapid test. ► Antibody prepared from modified Ag compared to unmodified A60 showed higher sensitivity, specificity, PPV and NPV. ► This minor modification of a commercially available test has led to a significant improvement in point-of-care testing of TB.