Whole genome sequencing of environmental Vibrio cholerae O1 from 10 nanograms of DNA using short reads

► Sequencing of DNA submitted to Multiple Displacement Amplification (MDA) may preclude genome assembly. ► We extracted genomic V. cholerae DNA in agarose block to ensure circularity. ► We MDA amplified 10 ng and assembled genome sequence de novo. ► Chimeras and spurious sequences were minimal.