Real-time PCR methods for quantitative monitoring of streptomycin and tetracycline resistance genes in agricultural ecosystems

► Development of qPCR multiplex assays for streptomycin and tetracycline resistance genes in plant and soil samples. ► Most frequently identified genes in soil pre- and post-slurry treatment were strB, aadA, tetB and tetM. ► Decrease in relative concentration of tetB and streptomycin resistance genes in samples taken post-slurry treatment.