DIY series of genetic cassettes useful in construction of versatile vectors specific for Alphaproteobacteria

►A DIY series of genetic cassettes has been developed. ►The cassettes facilitate construction of versatile vectors for Alphaproteobacteria. ►Insertion of a DIY cassette can change any plasmid to a mobilizable shuttle replicon functional in Alphaproteobacteria. ►A set of compatible E. coli-Alphaproteobacteria mobilizable shuttle vectors were created.