Immunomagnetic bead-based recovery and real time quantitative PCR (RT iq-PCR) for sensitive quantification of aflatoxin B1

► The first quantitative real-time immuno-PCR assay for aflatoxin B1. ► Several different approaches were examined (direct vs. indirect capture; mono- vs polyclonal antibody). ► Successfully quantification of trace levels of aflatoxin to help meet regulatory requirements. ► Assay may prove useful in food/feed analysis for foodborne/natural toxins or for detection of biological agents.