کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889848 | 1081506 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae](/preview/png/10889848.png)
چکیده انگلیسی
Haemophilus influenzae is an important human pathogen. A number of complete genome sequences of various haemophili are available; however, functional studies have been limited by the lack of an effective shuttle vector which functions in all strains. Here, we have constructed a shuttle vector, pEJ6, which transfers genes between Escherichia coli and H. influenzae and H. parainfluenzae. The vector contains an origin of replication from pLS88 which is functional in E. coli and H. influenzae. In addition it contains an RP4 mobilisation region. The vector can be introduced by electroporation and conjugation into capsulate and non-typeable H. influenzae and is functional for allelic replacement and mutant complementation. The vector will be useful for investigating gene function in Haemophilus spp.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 83, Issue 3, December 2010, Pages 330-334
Journal: Journal of Microbiological Methods - Volume 83, Issue 3, December 2010, Pages 330-334
نویسندگان
Esther Robinson, Mario Juhas, Derek Hood, Derrick Crook,