کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10890681 1081736 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of antimicrobial peptides from porcine neutrophils
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Analysis of antimicrobial peptides from porcine neutrophils
چکیده انگلیسی
Cationic host defence peptides are important components of innate immunity in pigs and other mammalians. Most of these peptides have a direct antimicrobial activity and they also have a broad spectrum of effects on the host immune system, which may be taken into account in the introduction of novel therapeutics. Our method permits simultaneous isolation of six antibacterial peptides, i.e. prophenin-1, prophenin-2, PR-39, and protegrins 1-3 from a porcine neutrophil crude extract and characterisation of them. Among the obtained peptides the greatest bactericidal activity expressed as MBC was seen in protegrins (10 μg/ml), whereas in the other studied peptides MBC was on the level of 20 μg/ml. Minimal inhibitory concentrations (MIC) reached 10 μg/ml for protegrins 1-3 and 20 μg/ml for prophenins, and PR-39. Within the bactericidal range all isolated peptides didn't show cytotoxicity on cell lines used in our experiment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 83, Issue 1, October 2010, Pages 8-12
نویسندگان
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