کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10890708 1081771 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimization of microbial DNA extraction and purification from raw wastewater samples for downstream pathogen detection by microarrays
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Optimization of microbial DNA extraction and purification from raw wastewater samples for downstream pathogen detection by microarrays
چکیده انگلیسی
Numerous waterborne pathogens are difficult to detect and enumerate with accuracy due to methodological limitations and high costs of direct culturing. The purity of DNA extracted from wastewater samples is an important issue in the sensitivity and the usefulness of molecular methods such as polymerase chain reaction (PCR) and hybridizations on DNA microarrays. Ten different DNA extraction procedures, including physical and chemical extraction and purification steps, were examined to ascertain their relative effectiveness for extracting bacterial DNA from wastewater samples. The quality of the differentially extracted DNAs was subsequently assessed by PCR amplification and microarray hybridization. Our results showed that great differences existed among the ten procedures and only a few of the methods gave satisfactory results when applied to bacterial pathogens. This observation suggested that the extraction method needed to be carefully selected to produce significant and confident results in the detection of pathogens from environmental samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 63, Issue 2, November 2005, Pages 115-126
نویسندگان
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