کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10890859 1081832 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A rapid microplate method for quantifying inhibition of bacterial adhesion to eukaryotic cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A rapid microplate method for quantifying inhibition of bacterial adhesion to eukaryotic cells
چکیده انگلیسی
Adhesion of bacteria to the mucosal epithelial cell surface is the first step in infection, and studies have shown that inhibition of this step may be useful therapeutically. To test compounds that may prevent bacterial binding to a number of epithelial cell lines, we have developed a high-throughput adhesion assay using a microtitre plate system and bacteria that have been modified to express firefly luciferase. This method has proved to be a sensitive, rapid, and reproducible system for screening antiadhesive agents for their effects on bacterial adhesion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 60, Issue 1, January 2005, Pages 55-62
نویسندگان
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