کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10892080 1082088 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Lipid content, active mitochondria and brilliant cresyl blue staining in bovine oocytes
ترجمه فارسی عنوان
محتوای لیپید، میتوکندری فعال و رنگ آمیزی کریسیل آبی در جوجه های گوشتی
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
Bovine oocytes that stain with brilliant cresyl blue (BCB) have a relatively higher developmental competence. The aim of the present study was to investigate the relationships among BCB staining, lipid content, and active mitochondria. Bovine oocytes (N = 133) with at least three layers of cumulus cells were segregated as BCB retained (BCB+) or metabolized (BCB-) and then stained for active mitochondria (Mitotracker Red) and lipid (Bodipy), with analysis by confocal microscopy. The BCB+ oocytes (N = 45) contained approximately 26% more cytoplasmic lipid than BCB- oocytes (N = 26-27; P < 0.05). Staining for active mitochondria did not differ between the groups. In BCB- oocytes but not BCB+ oocytes, lipid content correlated with active mitochondrial staining (r = 0.48; P < 0.05). Diameter correlated with lipid content for BCB+ oocytes (r = 0.46; P < 0.05), but not for BCB- oocytes (r = 0.16; P > 0.05). Irrespective of BCB staining, both lipid and active mitochondrial content correlated with diameter. In conclusion, the higher lipid content of BCB+ bovine oocytes might provide a cellular and functional basis for their greater developmental competence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 79, Issue 3, February 2013, Pages 417-422
نویسندگان
, , , , , , ,