کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10892105 | 1082088 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of fowl sperm motility: Evidence for the indirect, but not direct, involvement of dynein-ATPase activity on the reversible temperature-dependent immobilization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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![عکس صفحه اول مقاله: Regulation of fowl sperm motility: Evidence for the indirect, but not direct, involvement of dynein-ATPase activity on the reversible temperature-dependent immobilization Regulation of fowl sperm motility: Evidence for the indirect, but not direct, involvement of dynein-ATPase activity on the reversible temperature-dependent immobilization](/preview/png/10892105.png)
چکیده انگلیسی
Potential mechanisms of the reversible temperature-dependent immobilization of fowl sperm were investigated. At 30 °C, motility of demembranated fowl sperm was inhibited by adding 2 mM ethylene glycol-bis (2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA), but restored immediately after the subsequent addition of 2 mM CaCl2, whereas at 40 °C, such additions did not appreciably affect motility (which remained almost negligible). With intact sperm, 10â9 to 10â3 M Ca2+ had no effect on motility at 30 °C, which remained high. In contrast, intact sperm at 40 °C were almost immotile below 10â5 M Ca2+, and then gradually recovered motility at higher Ca2+ concentrations. The negligible motility of demembranated sperm at 40 °C, and at 30 °C in the presence of EGTA, was stimulated by addition of 100 nM of the protein phosphatase inhibitor calyculin A. Dynein-ATPase activities of sperm at 40 °C in the presence of 2 mM EGTA, 2 μM CaCl2, 2 mM CaCl2, or 100 nM calyculin A were higher than those at 30 °C. Therefore, stimulation of fowl sperm motility by temperature, Ca2+, and phosphatase inhibition was not simply associated with an increase of flagellar dynein-ATPase activity. Furthermore, Ca2+ was essential, at the axonemal level, for initiation of the 'intrinsic' motility of fowl sperm at 30 °C, but this Ca2+-dependent mechanism might be different from that involved in restoration of motility of intact sperm at 40 °C. In addition, perhaps inhibition of protein phosphatase activity was involved in initiation of sperm motility, but acting at a location different from Ca2+ on the axoneme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 79, Issue 3, February 2013, Pages 558-565
Journal: Theriogenology - Volume 79, Issue 3, February 2013, Pages 558-565
نویسندگان
Koji Ashizawa, Nao Oyama, Seiichi Katayama, Kazunori Narumi, Hideki Tatemoto, Yasuhiro Tsuzuki,