کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10893067 | 1082142 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Centrifugation on PureSperm® density-gradient improved quality of spermatozoa from frozen-thawed dog semen
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Centrifugation on PureSperm® density-gradient improved quality of spermatozoa from frozen-thawed dog semen Centrifugation on PureSperm® density-gradient improved quality of spermatozoa from frozen-thawed dog semen](/preview/png/10893067.png)
چکیده انگلیسی
The main objective of this study was to investigate if centrifugation through PureSperm® density-gradient can improve the post-thaw semen quality of dog semen. Semen from 5 dogs was collected and cryopreserved following a standard protocol. After thawing, semen samples were selected by centrifugation on PureSperm®. Assessments of sperm motility (assessed by computerized-assisted semen analysis), morphology (Diff-Quick staining) and viability (triple fluorescent stain of Propidium iodine/isothiocyanate-labeled peanut (Arachis hypogaea) agglutinin/Rhodamine 123), were performed on aliquots of fresh semen, unselected samples and selected preparations. Cryopreservation had a significant (P < 0.001) effect on all studied semen parameters. PureSperm® centrifugation yielded sperm suspensions with improved motility and viability (P < 0.001). The washing step significantly reduced (P < 0.001) all of the kinematics parameters evaluated as well as reduced the proportion of viable spermatozoa with intact acrosomes (P < 0.05). We concluded that PureSperm® centrifugation is a successful method for improving the quality of frozen-thawed dog spermatozoa. However, washing after density-gradient centrifugation dramatically reduces the post-thaw semen quality, indicating that the inclusion of such a washing step is unnecessary.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 76, Issue 2, 15 July 2011, Pages 381-385
Journal: Theriogenology - Volume 76, Issue 2, 15 July 2011, Pages 381-385
نویسندگان
J. Dorado, L. Alcaráz, N. Duarte, J.M. Portero, D. Acha, S. Demyda, A. Muñoz-Serrano, M. Hidalgo,