کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10893173 | 1082186 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of removal of necrotic blastomeres from mouse cryopreserved embryos on blastocyst formation and hatching
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
To evaluate whether the developmental potential of embryos that were partially damaged after freezing and thawing can be improved by removal of necrotic blastomeres. Eight-cell mouse embryos were cryopreserved using 1,2-propanediol and sucrose as cryoprotectant with slow cooling procedure. After thawing, blastocyst formation and hatching of fully intact embryos were compared between no treatment and with laser-assisted hatching. For partially intact embryos, the effects of removal of necrotic blastomeres with micromanipulation were evaluated. Laser-assisted hatching of mouse cryopreserved fully intact embryos significantly increased blastocyst hatching (63.4% versus 48.3%, PÂ <Â 0.05), but had little effect on blastocyst formation (72.0% versus 70.1%, PÂ >Â 0.05). The removal of necrotic blastomeres from partially damaged mouse cryopreserved embryos with micromanipulation significantly increased blastocyst formation (52.9% versus 32.0%, PÂ <Â 0.05) and blastocyst hatching (41.2% versus 22.0%, PÂ >Â 0.05) compared with the control group. The developmental potential of partially damaged cryopreserved embryos can be improved by removal of necrotic blastomeres with micromanipulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 64, Issue 5, 15 September 2005, Pages 1114-1120
Journal: Theriogenology - Volume 64, Issue 5, 15 September 2005, Pages 1114-1120
نویسندگان
Wei-Xin Liu, Yu Zheng, Meng-Jun Luo, Ping Huang, Li-Min Yue, Li Wang, Ya-Ping He, Jin-Hu Zhang,