کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10893219 | 1082187 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of breed on kinetics of ovine FSH and ovarian response in superovulated sheep
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Effects of breed on kinetics of ovine FSH and ovarian response in superovulated sheep Effects of breed on kinetics of ovine FSH and ovarian response in superovulated sheep](/preview/png/10893219.png)
چکیده انگلیسی
Embryo production is a useful tool for ex situ conservation of endangered species and breeds, despite a high variability in the ovarian response to superovulatory treatments. The current study evaluated the incidence and mechanisms of genetic factors in such variability, by determining the pharmacokinetics and pharmacodynamics of a standard treatment with ovine FSH (oFSH) in two endangered Spanish sheep breeds (Rubia del Molar, R, and Negra de Colmenar, N) in comparison to Manchega ewes (M, control group). In the first experiment, pharmacokinetics of an i.m. single dose of 1.32 mg of oFSH was evaluated in seven animals of each breed. Plasma FSH concentrations reached their maximum at 4 h post-administration in all the ewes, but several of the kinetic parameters (plasma FSH concentration at 4 h post-administration, maximum plasma FSH concentration, Cmax, and both the area under the plasma concentration-time curve extrapolated to the infinite, AUCinf, and to the last moment of sampling, AUClast) were higher in the N group. In the second trial, 10 animals of each breed were superovulated using eight decreasing doses of oFSH (3à 1.32 mg, 2à 1.10 mg, and 3à 0.88 mg). The R group, when compared to N and M, showed both a higher number of corpora lutea (13.7 ± 0.6 versus 10.0 ± 0.4 in N and 9.8 ± 0.6 in M, P < 0.05 for both) and embryos (7.9 ± 0.8 versus 4.3 ± 0.4 in N, P < 0.05, and 6.7 ± 0.5 in M, n.s.). Evaluation of pharmacokinetic and dynamic parameters showed that, although there was a trend for a higher hormone availability in R sheep, mean FSH plasma concentrations were similar between breeds (0.54 ± 0.08 ng/ml for R, 0.45 ± 0.05 ng/ml for N and 0.35 ± 0.05 ng/ml for M). However, differences were found in the number of preovulatory follicles growing in response to the FSH treatment between R (24.4 ± 2.2), M (18.9 ± 1.5, n.s.) and N sheep (14.1 ± 1.4; P < 0.01). Thus, differences in embryo yields between breeds would be related to differences in the pattern of follicular growth in response to FSH treatment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 66, Issue 4, 1 September 2006, Pages 896-905
Journal: Theriogenology - Volume 66, Issue 4, 1 September 2006, Pages 896-905
نویسندگان
I. Ammoun, T. Encinas, A. Veiga-Lopez, J.M. Ros, I. Contreras, P. Gonzalez-Añover, M.J. Cocero, A.S. McNeilly, A. Gonzalez-Bulnes,