کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10893797 1082234 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection
چکیده انگلیسی
The low efficiency of current microinjection-based animal transgenesis techniques is largely the result of poor embryo survival. We have developed a new, bacterial recombinase-based transgenesis method. Intracytoplasmic sperm injection (ICSI) of single stranded DNA (ssDNA) complexed with E. coli recombinase RecA into mouse metaphaseII (MII) arrested oocytes resulted in RecA-dependent transgenesis. This approach offers significant advantages over pronuclear microinjection and previous ICSI-based transgenesis approaches in terms of improved embryo survival, which translates into greater transgenesis efficiency. It also opens the possibility to attempt experiments, which may affect gene targeting by homologous recombination into DNA of mammalian single celled pre-implantation embryos.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 64, Issue 8, November 2005, Pages 1704-1715
نویسندگان
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