کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10900401 | 1084641 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neoxanthin and fucoxanthin induce apoptosis in PC-3 human prostate cancer cells
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کلمات کلیدی
FACSNeoxanthinDMEMTHFPARP3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromideDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMTT - MTTROS - ROSTetrahydrofuran - تتراهیدروفورانTdT-mediated dUTP nick end labeling - تلگراف پایان نام نهایی DUTP TdTTUNEL - تونلApoptosis - خزان یاختهایfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسProstate cancer cells - سلولهای سرطانی پروستاتFucoxanthin - فوکوکسانینpoly (ADP-ribose) polymerase - پلی (ADP-ribose) پلیمرازCarotenoid - کاروتنوئیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Neoxanthin and fucoxanthin, which have the characteristic structure of 5,6-monoepoxide and an allenic bond, were previously found to reduce the viability of human prostate cancer cells most intensively among 15 dietary carotenoids tested. In the present study, the induction of apoptosis in PC-3 cells by these two carotenoids was characterized by morphological changes, DNA fragmentation, an increased percentage of hypodiploid cells, and cleavages of caspase-3 and PARP. The ratio of apoptotic cells reached more than 30% after treatment for 48 h with 20 μM carotenoids. They reduced the expression of Bax and Bcl-2 proteins, but not Bcl-XL. Fucoxanthin accumulated in the cells at the same level as neoxanthin. Moreover, fucoxanthinol, a deacetylated product of fucoxanthin, formed in the cells treated with fucoxanthin and reached a level comparable to that of fucoxanthin after incubation for 24 h. Treatment by fucoxanthinol alone also induced apoptosis in PC-3 cells. Thus, neoxanthin and fucoxanthin treatments were found to induce apoptosis through caspase-3 activation in PC-3 human prostate cancer cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 220, Issue 1, 18 March 2005, Pages 75-84
Journal: Cancer Letters - Volume 220, Issue 1, 18 March 2005, Pages 75-84
نویسندگان
Eiichi Kotake-Nara, Akira Asai, Akihiko Nagao,