کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10912275 1088416 2005 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The large scale generation of dendritic cells for the immunization of patients with non-small cell lung cancer (NSCLC)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
The large scale generation of dendritic cells for the immunization of patients with non-small cell lung cancer (NSCLC)
چکیده انگلیسی
In the current study, we generated large numbers of dendritic cells (DCs) from patients with non-small cell lung cancer (NSCLC) for a vaccine trial. The DCs were generated from CD14+ cells obtained by immuno-magnetic bead column separation technique. The CD14+ cells were placed in culture in the presence of granulocyte macrophage colony stimulating factor (GMCSF) and Interleukin 4 (IL-4). At Day 7, apoptotic bodies derived from an allogeneic NSCLC line 1650-TC were added to the cultures at a DC:tumor cell ratio of 1:1. At Day 8, the DCs were harvested, washed and injected intradermally into patients. Using this protocol we have prepared DCs for 16 patients. An average of 9.3 × 107 DCs was injected for the priming dose and 8.2 × 107 DCs for the boost. Clinical evaluation of the patients and immune assessment are presented in a separate report. The current report provides evidence for the large scale production of functional DCs derived from patients with NSCLC which can be used as vaccines in clinical trials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Lung Cancer - Volume 47, Issue 3, March 2005, Pages 337-350
نویسندگان
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