کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926312 | 1091812 | 2012 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of Trpc channels, Stim1 and Orai1 in PGF2α-induced calcium signaling in NRK fibroblasts
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کلمات کلیدی
BHQ1-Oleoyl-2-acetyl-sn-glycerolReceptor-operated calcium entryROCENRKOrai1TRPCPGF2αOAGSOCESTIM1IP3inositol 1,4,5-trisphosphate - inositol 1،4،5-trisphosphateendoplasmic reticulum - شبکه آندوپلاسمی Stromal interaction molecule 1 - مولکول تعامل استروما 1Ca2+ oscillation - نوسانات Ca2 +Store-operated calcium entry - واردات کلسیم در فروشگاهProstaglandin F2α - پروستاگلاندین F2αTransient receptor potential channels - کانال های بالقوه گیرنده گذراnormal rat kidney - کلیه موش طبیعی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Normal rat kidney (NRK) fibroblasts exhibit growth-dependent changes in electrophysiological properties and intracellular calcium dynamics. The transition from a quiescent state to a density-arrested state results in altered calcium entry characteristics. This coincides with modulation of the expression of the genes encoding the calcium channels Trpc1, Trpc6 and Orai1, and of the intracellular calcium sensor Stim1. In the present study we have used gene selective short hairpin (sh) RNAs against these various genes to investigate their role in (a) capacitative store-operated calcium entry (SOCE); (b) non-capacitative OAG-induced receptor-operated calcium entry (ROCE); and (c) prostaglandin F2α (PGF2α)-induced Ca2+-oscillations in NRK fibroblasts. Intracellular calcium measurements revealed that knockdown of the genes encoding Trpc1, Orai1 and Stim1 each caused a significant reduction of SOCE in NRK cells, whereas knockdown of the gene encoding Trpc6 reduced only the OAG-induced ROCE. Furthermore, our data show that knockdown of the genes encoding Trpc1, Orai1 and Stim1, but not Trpc6, substantially reduced the frequency (up to 60%) of PGF2α-induced Ca2+ oscillations in NRK cells. These results indicate that in NRK cells distinct calcium channels control the processes of SOCE, ROCE and PGF2α-induced Ca2+ oscillations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 51, Issue 1, January 2012, Pages 12-21
Journal: Cell Calcium - Volume 51, Issue 1, January 2012, Pages 12-21
نویسندگان
W.H.M. Almirza, P.H.J. Peters, E.J.J. van Zoelen, A.P.R. Theuvenet,