کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926486 | 1091856 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nuclear calcium signaling by inositol trisphosphate in GH3 pituitary cells
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کلمات کلیدی
2-APBinositol trisphosphate receptorsAEqIP3HSVDAPIGFPinositol-1,4,5-trisphosphate - inositol-1،4،5-trisphosphate[Ca2+]c - [Ca2 +] cAequorin - اکوورینtrh - بازارdiamidino-2-phenylindole - دیامیدینو 2-فنیلینولPituitary cells - سلول های هیپوفیزendoplasmic reticulum - شبکه آندوپلاسمی Nucleoplasmic reticulum - شبکیه نوکلئوپلاسمیکCytosolic Ca2+ concentration - غلظت Ca2 + سیتواستولیthyrotropin-releasing hormone - هورمون آزاد کننده تیروتروپینherpes simplex virus - ویروس هرپس سیمپلکسnuclear envelope - پاکت هسته ایgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It has been proposed that nuclear and cytosolic Ca2+ ([Ca2+]N and [Ca2+]C) may be regulated independently. We address here the issue of whether inositol trisphosphate (IP3) can, bypassing changes of [Ca2+]C, produce direct release of Ca2+ into the nucleoplasm. We have used targeted aequorins to selectively measure and compare the changes in [Ca2+]C and [Ca2+]N induced by IP3 in GH3 pituitary cells. Heparin, an IP3 inhibitor that does not permeate the nuclear pores, abolished the [Ca2+]C peaks but inhibited only partly the [Ca2+]N peaks. The permeant inhibitor 2-aminoethoxy-diphenyl-borate (2-APB) blocked both responses. Removal of ATP also inhibited more strongly the [Ca2+]C than [Ca2+]N peak. The [Ca2+]N and [Ca2+]C responses differed also in their sensitivity to IP3, the nuclear response showing higher affinity. Among IP3 receptors, type 2 (IP3R2) has a higher affinity for IP3 and is not inactivated by ATP removal. We find that IP3R2 immunoreactivity is present inside the nucleus whereas the other IP3R subtypes are detected only in the cytoplasm. The nuclear envelope (NE) of GH3 cells showed deep invaginations into the nucleoplasm, with cytosol and cytoplasmic organella inside. These results indicate that GH3 pituitary cells possess mechanisms able to produce selective increases of [Ca2+]N.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 43, Issue 2, February 2008, Pages 205-214
Journal: Cell Calcium - Volume 43, Issue 2, February 2008, Pages 205-214
نویسندگان
Pablo Chamero, Isabel M. Manjarres, José Manuel GarcÃa-Verdugo, Carlos Villalobos, Maria Teresa Alonso, Javier GarcÃa-Sancho,