کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10928010 | 1092889 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cryopreservation of putative pre-pubertal bovine spermatogonial stem cells by slow freezing
ترجمه فارسی عنوان
غربالگری احتمالا سلول های بنیادی اسپرماتوگونیال گاوی قبل از بلوغ توسط انجماد آهسته
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کلمات کلیدی
گاو، فریزر انجماد آهسته، سلول بنیادی اسپرماتوگونال، ترهالوز،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
Development of techniques for the preservation of mammalian spermatogonial stem cells (SSCs) is a critical step in commercial application of SSC based technologies, including species preservation, amplification of agriculturally valuable germ lines, and human fertility preservations. The objective of this study was to develop an efficient cryopreservation protocol for preservation of bovine SSCs using a slow freezing technique. To maximize the efficiency of SSC cryopreservation, the effects of various methods (tissue vs. cell freezing) and cryoprotective agents (trehalose, sucrose, and polyethylene glycol [PEG]) were tested. Following thawing, cells were enriched for undifferentiated spermatogonia by differential plating and evaluated for recovery rate, proliferation capacity, and apoptosis. Additionally, putative stem cell activity was assessed using SSC xenotransplantation. The recovery rate, and proliferation capacity of undifferentiated spermatogonia were significantly greater for germ cells frozen using tissue freezing methods compared to cell freezing methods. Cryopreservation in the presence of 200Â mM trehalose resulted in significantly greater recovery rate, proliferation capacity, and apoptosis of germ cells compared to control. Furthermore, cryopreservation using the tissue freezing method in the presence of 200Â mM trehalose resulted in the production of colonies of donor-derived germ cells after xenotransplantation into recipient mouse testes, indicating putative stem cell function. Collectively, these data indicate that cryopreservation using tissue freezing methods in the presence of 200Â mM trehalose is an efficient cryopreservation protocol for bovine SSCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 70, Issue 2, April 2015, Pages 175-183
Journal: Cryobiology - Volume 70, Issue 2, April 2015, Pages 175-183
نویسندگان
Ki-Jung Kim, Yong-An Lee, Bang-Jin Kim, Yong-Hee Kim, Byung-Gak Kim, Hyun-Gu Kang, Sang-Eun Jung, Sun-Ho Choi, Jonathan A. Schmidt, Buom-Yong Ryu,