Cooling rate dependent biophysical and viability response shift with attachment state in human dermal fibroblast cells

► Celgosivir is an effective nanomolar concentration inhibitor against all four dengue virus serotypes. ► Celgosivir effectively inhibits DENV-2 in ADE infected THP-1 cells. ► NS1 protein misfolds and accumulates in the ER during celgosivir treatment. ► Celgosivir treatment promotes the expression of pro-survival ER stress response proteins. ► Celgosivir treatment is effective up 48 h post-virus infection in a lethal challenge mouse model.