کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10928492 | 1092904 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitrification of in vitro produced bovine embryos: Effect of embryonic block and developmental kinetics
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کلمات کلیدی
Me2SOTCNtrophectodermCOCICMEGAEthylene glycol - اتیلن گلیکولInner cell mass - توده سلولی داخلیEmbryo - جنینRoom temperature - دمای اتاقDimethyl sulfoxide - دیمتیل سولفواکسیدvitrification solution - راه حل انجمادKinetics - سینتیک (جنبش شناسی) Vitrification - شیشهشدگیembryonic genome activation - فعال سازی ژنوم جنینیcumulus oocyte complex - پیچیده ی تخم مرغی کومولوسBovine - گاو
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Vitrification of in vitro produced bovine embryos: Effect of embryonic block and developmental kinetics Vitrification of in vitro produced bovine embryos: Effect of embryonic block and developmental kinetics](/preview/png/10928492.png)
چکیده انگلیسی
In order to investigate whether the kinetics and stage of embryo development affect cryosurvival of in vitro produced bovine embryos, cleaved embryos were categorized in six groups based on their developmental kinetics regarding the stage of embryonic block in bovine (8-16 cell stage): I and II - early (day 2) and late (day 3) 5-8 cell, III and IV - early (day 3) and late (day 4) 8-16 cell, and V and VI - early (day 4) and late (day 5) morula. The cryosurvival and developmental competence of these embryos were compared with each other and also with the corresponding control groups. The potential of 5-8 cell stage embryos to survive vitrification and further develop towards blastocyst stage was significantly lower than vitrified and un-vitrified 8-16 cell and morula stage embryos. These results suggest that, the survival rate and potential of embryos to develop towards blastocyst stage might be affected by the kinetic of the embryo development. Moreover, the results of this study indicated that the optimal stages of early embryo vitrification are post-embryonic block.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 65, Issue 3, December 2012, Pages 278-283
Journal: Cryobiology - Volume 65, Issue 3, December 2012, Pages 278-283
نویسندگان
V. Asgari, S.M. Hosseini, M. Forouzanfar, M. Hajian, M.H. Nasr-Esfahani,