کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10928828 | 1092958 | 2005 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phospholipid vesicles increase the survival of freeze-dried human red blood cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Phospholipid vesicles increase the survival of freeze-dried human red blood cells Phospholipid vesicles increase the survival of freeze-dried human red blood cells](/preview/png/10928828.png)
چکیده انگلیسی
In a previous report [Z. Török, G. Satpathy, M. Banerjee, R. Bali, E. Little, R. Novaes, H. Van Ly, D. Dwyre, A. Kheirolomoom, F. Tablin, J.H. Crowe, N.M. Tsvetkova, Preservation of trehalose loaded red blood cells by lyophilization, Cell Preservation Technol. 3 (2005) 96-111.], we presented a method for preserving human red blood cells (RBCs) by loading them with trehalose and then freeze-drying. We have now improved that method, based on the discovery that addition of phospholipid vesicles to the lyophilization buffer substantially reduces hemolysis of freeze-dried RBCs after rehydration. The surviving cells synthesize 2,3-DPG, have low levels of methemoglobin, and have preserved morphology. Among the lipid species we studied, unsaturated PCs were found to be most effective in suppressing hemoglobin leakage. RBC-vesicle interactions depend on vesicle size and structure; unilamellar liposomes with average diameter of less than 300 nm were more effective in reducing the hemolysis than multilamellar vesicles. Trehalose loaded RBCs demonstrated high survival and low levels of methemoglobin during 10 weeks of storage at 4 °C in the dry state when lyophilized in the presence of liposomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 51, Issue 3, December 2005, Pages 290-305
Journal: Cryobiology - Volume 51, Issue 3, December 2005, Pages 290-305
نویسندگان
Azadeh Kheirolomoom, Gyana R. Satpathy, Zsolt Török, Mitali Banerjee, Rachna Bali, Roberta C. Novaes, Erika Little, Danielle M. Manning, Denis M. Dwyre, Fern Tablin, John H. Crowe, Nelly M. Tsvetkova,