کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10931928 | 1093679 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
NANOS2 promotes male germ cell development independent of meiosis suppression
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کلمات کلیدی
PBSStra8PGCDKO5-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineRNA immunoprecipitation - ایمن سازی RNABrdU - بروموداکسی اوریدینImmunoprecipitation - تخریب ایمنیRetinoic acid - رتینوئیک اسیدembryonic day - روز جنینیreverse transcription - رونویسی معکوسMale germ cell - سلول های بنیادیPrimordial germ cell - سلول های بنیادی اولیهPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریdouble knockout - نابودی دوگانهNanos2 - نانوس 2polymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازRIP - پاره کردن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
NANOS2 is an RNA-binding protein essential for fetal male germ cell development. While we have shown that the function of NANOS2 is vital for suppressing meiosis in embryonic XY germ cells, it is still unknown whether NANOS2 plays other roles in the sexual differentiation of male germ cells. In this study, we addressed the issue by generating Nanos2/Stra8 double knockout (dKO) mice, whereby meiosis was prohibited in the double-mutant male germ cells. We found that the expression of male-specific genes, which was decreased in the Nanos2 mutant, was hardly recovered in the dKO embryo, suggesting that NANOS2 plays a role in male gene expression other than suppression of meiosis. To investigate the molecular events that may be controlled by NANOS2, we conducted a series of microarray analyses to search putative targets of NANOS2 that fulfilled 2 criteria: (1) increased expression in the Nanos2 mutant and (2) the mRNA associated with NANOS2. Interestingly, the genes predominantly expressed in undifferentiated primordial germ cells (PGCs) were significantly selected, implying the involvement of NANOS2 in the termination of the characteristics of PGCs. Furthermore, we showed that NANOS2 is required for the maintenance of mitotic quiescence, but not for the initiation of the quiescence in fetal male germ cells. These results suggest that NANOS2 is not merely a suppressor of meiosis, but instead plays pivotal roles in the sexual differentiation of male germ cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 385, Issue 1, 1 January 2014, Pages 32-40
Journal: Developmental Biology - Volume 385, Issue 1, 1 January 2014, Pages 32-40
نویسندگان
Rie Saba, Yuzuru Kato, Yumiko Saga,