The functional role of the Meis/Prep-binding elements in Pax6 locus during pancreas and eye development

► Genomic deletion analysis identifies an essential pancreatic enhancer for Pax6. ► The Pax6 ectodermal enhancer cooperates with its adjacent sequences to regulate Pax6 lens expression. ► Prep1 is dispensable for the maintenance of Pax6 lens and pancreas expression.