Time-lapse imaging reveals stereotypical patterns of Drosophila midline glial migration

► Multiple imaging approaches describe Drosophila CNS midline glial development. ► Different groups of midline glia collectively migrate in different directions. ► Midline glia migrate to specific positions surrounding the axon commissures. ► Suggests the existence of specific cues that guide the migration of midline glia. ► Individual midline glia extend elaborate membrane processes.