کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10933482 1093799 2009 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The regulatory mechanisms that underlie inappropriate transcription of the myogenic determination gene Myf5 in the central nervous system
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
The regulatory mechanisms that underlie inappropriate transcription of the myogenic determination gene Myf5 in the central nervous system
چکیده انگلیسی
Myf5 is a key myogenic determination factor, specifically present at sites of myogenesis. Surprisingly, during mouse development, this gene is also transcribed in restricted areas of the central nervous system, although the Myf5 protein is not detectable. We have investigated the regulation of Myf5 expression in the central nervous system. Using both in ovo electroporation in the chick embryo and transgenesis in the mouse, we show that regulatory sequences that direct neuronal Myf5 transcription are present in a distal element located between − 55 and − 54.3 Kb from the Myf5 gene. An Oct6/Tst1 binding site is required for embryonic brain expression, and in the Oct6 mutant mouse embryo, Myf5 transcripts are no longer detectable in the brain. The Wnt-β catenin signalling pathway is also implicated. Finally we show that post-transcriptional regulation of Myf5 gene expression involves miRNA repression acting through the Myf5-3′UTR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 327, Issue 1, 1 March 2009, Pages 71-82
نویسندگان
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