کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10934004 1093876 2005 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Calcium signaling differentiation during Xenopus oocyte maturation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Calcium signaling differentiation during Xenopus oocyte maturation
چکیده انگلیسی
Ca2+ is the universal signal for egg activation at fertilization in all sexually reproducing species. The Ca2+ signal at fertilization is necessary for egg activation and exhibits specialized spatial and temporal dynamics. Eggs acquire the ability to produce the fertilization-specific Ca2+ signal during oocyte maturation. However, the mechanisms regulating Ca2+ signaling differentiation during oocyte maturation remain largely unknown. At fertilization, Xenopus eggs produce a cytoplasmic Ca2+ (Ca2+cyt) rise that lasts for several minutes, and is required for egg activation. Here, we show that during oocyte maturation Ca2+ transport effectors are tightly modulated. The plasma membrane Ca2+ ATPase (PMCA) is completely internalized during maturation, and is therefore unable to extrude Ca2+ out of the cell. Furthermore, IP3-dependent Ca2+ release is required for the sustained Ca2+cyt rise in eggs, showing that Ca2+ that is pumped into the ER leaks back out through IP3 receptors. This apparent futile cycle allows eggs to maintain elevated cytoplasmic Ca2+ despite the limited available Ca2+ in intracellular stores. Therefore, Ca2+ signaling differentiates in a highly orchestrated fashion during Xenopus oocyte maturation endowing the egg with the capacity to produce a sustained Ca2+cyt transient at fertilization, which defines the egg's competence to activate and initiate embryonic development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 288, Issue 2, 15 December 2005, Pages 514-525
نویسندگان
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