کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10940214 | 1095469 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rbm19 is a nucleolar protein expressed in crypt/progenitor cells of the intestinal epithelium
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کلمات کلیدی
B23C23Colorectal adenocarcinoma - آدنوکارسینوم کولورکتالOrganogenesis - اندام زایی، ارگانوژنزDifferentiation - تفکیکDevelopment - رشدIntestine - رودهIntestinal Crypt - روده رودهRibosome biogenesis - زیست زیستی ریبوسومColon cancer - سرطان کولونStem cell - سلول بنیادیCaco-2 cells - سلول های Caco-2Morphogenesis - مورفوژنزMitosis - میتوزNucleolin - نولولینnucleophosmin - نوکلئوفسمیNucleolus - هستکRNA-binding proteins - پروتئین های مرتبط با RNAprogenitor - پیشگام
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Intestinal development and homeostasis rely on the coordination of proliferation and differentiation of the epithelium. To better understand this process, we are studying Rbm19, a gene expressed in the gut epithelium that is essential for intestinal morphogenesis and differentiation in the zebrafish (Development 130, 3917). Here we analyzed the expression of Rbm19 in several biological contexts that feature proliferation/differentiation cell fate decisions. In the undifferentiated embryonic gut tube, Rbm19 is expressed throughout the epithelium, but then becomes localized to the crypts of Lieberkühn of the adult intestine. Consistent with its expression in adult crypt/progenitor cells, expression is widespread in human colorectal carcinomas and dividing Caco-2 cells. Its expression in Caco-2 cells recapitulates the in vivo pattern, declining when the cells undergo confluence-induced arrest and differentiation. Rbm19 protein localizes to the nucleolus during interphase and to the perichromosomal sheath during mitosis, in accordance with the pattern described for other nucleolar proteins implicated in ribosome biogenesis. Interestingly, the loss of nucleolar rbm19, nucleolin/C23, and nucleophosmin/B23 in confluent Caco-2 cells did not signify loss of nucleoli as detected by electron microscopy. Taken together, these data point to the nucleolus as a possible locus for regulating the proliferation/differentiation cell fate decision in the intestinal epithelium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 6, Issue 1, December 2005, Pages 45-56
Journal: Gene Expression Patterns - Volume 6, Issue 1, December 2005, Pages 45-56
نویسندگان
James A. Lorenzen, Benedetta B. Bonacci, Rachel E. Palmer, Clive Wells, Jian Zhang, Daniel A. Haber, Allan M. Goldstein, Alan N. Mayer,