کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10954013 | 1097830 | 2010 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polyol pathway impairs the function of SERCA and RyR in ischemic-reperfused rat hearts by increasing oxidative modifications of these proteins
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کلمات کلیدی
SDHRyRRLULVDPGAPDHGSHLVEDPNADPHLVSPsorbitol dehydrogenaset50S-GlutathiolationSDINCXARII/R - I / RNa+/Ca2+ exchanger - Na + / Ca2 + مبدلNAD+ - NAD +NADP+ - NADP +ROS - ROS[Ca2+]i - [Ca2 +] iAdenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPAldose reductase - آلدووز ردوکتازMyocardial ischemia and reperfusion - ایسکمی میوکارد و reperfusionischemia–reperfusion - ایسکمی-رپرفیوژنLeft ventricular developed pressure - بطن چپ توسعه یافته فشارOxidative stress - تنش اکسیداتیوsham-operated - شام کار می کندHeart rate - ضربان قلبleft ventricular systolic pressure - فشار سیستولیک بطن چپleft ventricular end-diastolic pressure - فشار پای دیاستولیک بطن چپSERCA - قلبlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH polyol pathway - مسیر پلیولAldose reductase inhibitor - مهار کننده آلدوز ردوکتازNADH - نادانTyrosine nitration - نیتراسیون تیروزینnitrotyrosine - نیتروتیروستینnicotinamide adenine dinucleotide - نیکوتین آمید adenine dinucleotidenicotinamide adenine dinucleotide phosphate - نیکوتین آمید adenine dinucleotide phosphaterelative light unit - واحد نسبی نورreduced nicotinamide adenine dinucleotide - کاهش ninocotinamide adenine dinucleotidereduced nicotinamide adenine dinucleotide phosphate - کاهش نیکوتین آمید آدنین دینکلوتید فسفاتIntracellular calcium - کلسیم داخل سلولیGlutathione - گلوتاتیونglyceraldehydes-3-phosphate dehydrogenase - گلیسرالید هیدروژن 3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژنRyanodine receptor - گیرنده رایانودین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Polyol pathway impairs the function of SERCA and RyR in ischemic-reperfused rat hearts by increasing oxidative modifications of these proteins Polyol pathway impairs the function of SERCA and RyR in ischemic-reperfused rat hearts by increasing oxidative modifications of these proteins](/preview/png/10954013.png)
چکیده انگلیسی
A number of studies have shown that the polyol pathway, consisting of aldose reductase (AR) and sorbitol dehydrogenase (SDH), contributes to ischemia-reperfusion (I/R)-induced myocardial infarction due to depletion of ATP. In this report we show that the polyol pathway in I/R heart also contributes to the impairment of sacro/endoplasmic reticulum Ca2+-ATPase (SERCA) and ryanodine receptor (RyR), two key players in Ca2+ signaling that regulate cardiac contraction. Rat hearts were isolated and retrogradely perfused with either Krebs' buffer containing 1 μM AR inhibitor, zopolrestat, or 200 nM SDH inhibitor, CP-170,711, and challenged by 30 min of regional ischemia and 45 min of reperfusion. We found that post-ischemic contractile function of the isolated perfused hearts was improved by pharmacological inhibition of the polyol pathway. I/R-induced contractile dysfunction is most likely due to impairment in Ca2+ signaling and the activities of SERCA and RyR. All these abnormalities were significantly ameliorated by treatment with ARI or SDI. We showed that the polyol pathway activities increase the level of peroxynitrite, which enhances the tyrosine nitration of SERCA and irreversibly modifies it to form SERCAC674-SO3H. This leads to reduced level of S-glutathiolated SERCA, contributing to its inactivation. The polyol pathway activities also deplete the level of GSH, leading to decreased active RyR, the S-glutathiolated RyR. Thus, in I/R heart, inhibition of polyol pathway improved the function of SERCA and RyR by protecting them from irreversible oxidation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 49, Issue 1, July 2010, Pages 58-69
Journal: Journal of Molecular and Cellular Cardiology - Volume 49, Issue 1, July 2010, Pages 58-69
نویسندگان
Wai Ho Tang, Gennadi M. Kravtsov, Martina Sauert, Xiao Yong Tong, Xiu Yun Hou, Tak Ming Wong, Sookja K. Chung, Stephen Sum Man Chung,