کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956820 | 1099458 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Direct interaction of N-ethylmaleimide-sensitive factor with GABAA receptor β subunits
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کلمات کلیدی
Receptor for activated C kinaseGSTRACK-1Hap1NSFAMPApKatype A GABA receptorα-amino-3-hydroxy-5-methyl-4-isoxazole propionic acidPKCγ-aminobutyric acid - اسید γ-آمینوبوتیریکEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاN-ethylmaleimide-sensitive factor - عامل حساس N-ethylmaleimideprotein kinase A - پروتئین کیناز AProtein kinase C - پروتئین کیناز سیGABA - گاباGABARAP - گاباراپglutathione S-transferase - گلوتاتیون S-ترانسفرازGABAA receptor - گیرنده GABAA یا گیرنده گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
GABAA receptors mediate most of the fast inhibitory neurotransmission in the brain, and are believed to be composed mainly of α, β, and γ subunits. It has been shown that GABAA receptors interact with a number of binding partners that act to regulate both receptor function and cell surface stability. Here, we reveal that GABAA receptors interact directly with N-ethylmaleimide-sensitive factor (NSF), a critical regulator of vesicular dependent protein trafficking, as measured by in vitro protein binding and co-immunoprecipitation assays. In addition, we established that NSF interacts with residues 395-415 of the receptor β subunits and co-localizes with GABAA receptors in hippocampal neurons. We also established that NSF can regulate GABAA receptor cell surface expression depending upon residues 395-415 in the β3 subunit. Together, our results suggest an important role for NSF activity in regulating the cell surface stability of GABAA receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 30, Issue 2, October 2005, Pages 197-206
Journal: Molecular and Cellular Neuroscience - Volume 30, Issue 2, October 2005, Pages 197-206
نویسندگان
Hidefumi Goto, Miho Terunuma, Takashi Kanematsu, Yoshio Misumi, Stephen J. Moss, Masato Hirata,