Exploring long non-coding RNAs through sequencing

► RNA-seq and other NGS-based approaches enable the analysis of non-coding transcriptomes. ► lncRNAs can be transcribed from promoters of coding transcripts, enhancers, repetitive elements, antisense to protein-coding genes, or separate from coding genes. ► lncRNAs are transcriptionally and post-transcriptionally regulated. ► Specific and dynamic lncRNA expression patterns and subcellular localisations provide insights into their functions.