کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10971503 1106474 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reciprocal interaction between fish TGF-β1 and IL-1β is responsible for restraining IL-1β signaling activity in grass carp head kidney leukocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Reciprocal interaction between fish TGF-β1 and IL-1β is responsible for restraining IL-1β signaling activity in grass carp head kidney leukocytes
چکیده انگلیسی
In the present study, we found that recombinant grass carp IL-1β (rgcIL-1β) simultaneously up-regulated grass carp IL-1β (gcIL-1β) and TGF-β1 (gcTGF-β1) expression via NF-κB and MAPK signaling in grass carp head kidney leukocytes (HKLs), promoting us to clarify whether TGF-β1 is an effective antagonist in IL-1β expression and activity. Our results showed that a stimulation of gcIL-1β on its own expression was noted within 6 h, but gcTGF-β1 neutralizing antibody prolonged gcIL-1β autostimulation up to 12 h, indicating a possible inhibitory role of gcTGF-β1 in regulating gcIL-1β effect. This notion was reinforced by the fact that recombinant grass carp TGF-β1 (rgcTGF-β1) could impede rgcIL-1β-induced gcIL-1β gene expression and secretion in a reciprocal manner. Further studies revealed that rgcTGF-β1 was able to attenuate rgcIL-1β-induced mRNA expression of its own receptor signaling molecules and the activation of NF-κB. By contrast, rgcIL-1β significantly amplified rgcTGF-β1-mediated gcTGF-β1 type I receptor (ALK5) expression and Smad2 phosphorylation in the same cell model. Taken together, these data shed light on an intrinsic mechanism for controlling inflammatory response by the reciprocal interaction between TGF-β1 and IL-1β in teleost.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 47, Issue 2, December 2014, Pages 197-204
نویسندگان
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