Molecular cloning and partial functional characterization of a proliferation inducing ligand (APRIL) in zebrafish (Danio rerio)

► We successfully amplified the cDNA of Père David's deer BAFF (miBAFF) from peripheral blood. ► Phylogenetic analysis exhibited this sequence has high homology with the mammals especially cow. ► We constructed fusion protein of the pSUMO-misBAFF and obtained purity misBAFF. ► The fusion protein misBAFF represents a readily obtainable source of biologically activity.